Genome editing has revolutionized the way scientists can edit DNA sequences in various living organisms. CRISPR, which stands for clustered regularly interspaced short palindromic repeats, is a fast, reliable, and efficient technology for site-directed genome editing compared to early methods such as zinc finger nuclease (ZFNs) or transcription activator-like effector nucleases (TALENS).
Antisense oligos can be used in conjunction with RNaseH to digest DNA–rRNA hybrids (5, 24). Several studies in both mammals and bacteria have shown that RNaseH-mediated rRNA depletion is efficient, resulting in sequencing libraries with minimal rRNA derived reads ( 5 , 23–27 ).
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what is antisense dna